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Description
Research Area
DNA and RNA Research, Transcription Factors
Images & Validation
−Item 1 of 2
| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Zebrafish |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human eEF1A2 (1-100/463aa) |
| Target | EEF1A2 |
| Molecular Weight | 53 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DEE33; EEF1AL; EF-1-alpha-2; EF1A; EIEE33; HS1; MRD38; STN; STNL; Eef1a; S1; wasted; wst; Ps10; RATPS10; XELAEV_18046315mg; eef1a2; EF1A2_HUMAN; Eukaryotic elongation factor 1 A-2 (eEF1A-2); Statin-S1; 3.6.5.-; EF1A2_MOUSE; EF1A2_RAT; eukaryotic translation elongation factor 1 alpha 2; statin-like; statin
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25 ug total protein per lane of various lysates probed with eEF1A2 polyclonal antibody, unconjugated (orb156698) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (eEF1A2) Polyclonal Antibody, Unconjugated (orb156698) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Quick Database Links
Gene Symbol
EEF1A2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
EEF1A2 Rabbit Polyclonal Antibody (orb156698)
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