ECH1 Rabbit Polyclonal Antibody

SKU: orb156686

Description

ECH1 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Markers, Fatty Acid Oxidation, Fatty Acids, Lipid Metabolism, Metabolism, Mitochondrial Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ECH1 (21-120/328aa)
• Target: ECH1
• Molecular Weight: 32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

4)-dienoyl-CoA isomerase; 5)-delta(2; delta(3 5) delta(2 4) dienoyl CoA isomerase; delta(3 5) delta(2 4) dienoyl CoA isomerase mitochondrial; delta(3; delta3 5 delta2 4 dienoyl CoA isomerase; Dienoyl CoA isomerase; ECH 1; Ech1; ECH1_HUMAN; Enoyl Coenzyme A hydratase 1; Enoyl Coenzyme A hydratase 1 peroxisomal; HPXEL; mitochondrial; MS1034; Peroxisomal enoyl CoA hydratase 1; Peroxisomal enoyl coenzyme A hydratase like protein.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ECH1) Polyclonal Antibody, Unconjugated (orb156686) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ECH1

UniProt

Q13011