E1 Ubiquitin Antibody

• Catalog Number: orb1264991
• Category: Antibodies
• Description: E1 Ubiquitin Antibody
• Target: UBA1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rabbit, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This E1 Ubiquitin (UBE1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1026-1058 amino acids from the C-terminal region of human E1 Ubiquitin (UBE1).
• UniProt ID: P22314
• MW: 118 kDa
• Tested applications: IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Ubiquitin-like modifier-activating enzyme 1, Prote
• Note: For research use only
• NCBI: P22314

Western blot analysis of E1 Ubiquitin (UBE1) Antibody in mouse stomach tissue lysates (35 ug/lane)

Antibody is used in Western blot to detect UBE1 in HL-60 cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with UBE1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.