E-Selectin rabbit pAb

• Catalog Number: orb771338
• Category: Antibodies
• Description: Rabbit polyclonal antibody to E-selectin
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from the N-terminal region of human SELE. AA range:100-150
• Concentration: 1 mg/ml
• Dilution range: IHC-p: 100-300.WB 1:500-2000, ELISA 1:10000-20000
• Conjugation: Unconjugated
• MW: 66
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-selectin E antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of K562 SH-SY5Y mouse-brain cells using E-Selectin Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000.

Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.

Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).