E. coli LACI protein

• Catalog Number: orb392046
• Category: Proteins
• Description: Recombinant e. coli LACI protein is produced by our E. coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: E. coli
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT, pH 8.0.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: LACI
• Entrez: 945007
• UniProt ID: P03023
• Protein Sequence: MKPVTLYDVA EYAGVSYQTV SRVVNQASHV SAKTREKVEA AMAELNYIPN RVAQQLAGKQ SLLIGVATSS LALHAPSQIV AAIKSRADQL GASVVVSMVE RSGVEACKAA VHNLLAQRVS GLIINYPLDD QDAIAVEAAC TNVPALFLDV SDQTPINSII FSHEDGTRLG VEHLVALGHQ QIALLAGPLS SVSARLRLAG WHKYLTRNQI QPIAEREGDW SAMSGFQQTM QMLNEGIVPT AMLVANDQMA LGAMRAITES GLRVGADISV VGYDDTEDSS CYIPPLTTIK QDFRLLGQTS VDRLLQLSQG QAVKGNQLLP VSLVKRKTTL APNTQTASPR ALADSLMQLA RQVSRLESGQ HHHHHH
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT, pH 8.0.
• Alternative names: Lactose operon repressor
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.