Dystrobrevin alpha Rabbit Polyclonal Antibody

• Catalog Number: orb500557
• Category: Antibodies
• Description: Dystrobrevin alpha Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Canine, Equine
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse Dystrobrevin alpha (301-400/743aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 81 kDa
• Target: DTNA
• UniProt ID: Q9D2N4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: DRP3; DTN; DTNA; Dystrophin related protein 3; FLJ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Dystrobrevin alpha) Polyclonal Antibody, Unconjugated (orb500557) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Dystrobrevin alpha) polyclonal Antibody, Unconjugated (orb500557) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.