DYKDDDDK Tag Rabbit Polyclonal Antibody

SKU: orb1294275

Description

Rabbit polyclonal antibody to DYKDDDDK tag

Images & Validation

• Tested Applications: WB
• Dilution Range: Western Blot 1:3000-1:5000, Immunofluorescence 1:500-1:1000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide (DYKDDDDK)
• Target: DYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti DYKDDDDK-tag antibody at 1/3000 dilution, Lysates/proteins at 35 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294275 overnight at 4°C.

Quick Database Links

Gene Symbol

DYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2)