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DYKDDDDK Tag (same epitope as Sigma's FLAG M2) Antibody
Description
Images & Validation
−| Tested Applications | WB |
|---|---|
| Dilution range | Western Blot 1:3000-1:5000 Immunofluorescence 1:500-1:1000 |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide (DYKDDDDK) |
| Target | DYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2) |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Disclaimer | For research use only |

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All lanes: Anti DYKDDDDK-tag antibody at 1/3000 dilution, Lysates/proteins at 35 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294275 overnight at 4°C.
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DYKDDDDK Tag (same epitope as Sigma's FLAG M2) Antibody (orb1294275)
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