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DYKDDDDK Tag (same epitope as Sigma's FLAG M2) Antibody

SKU: orb1294275

Description

Rabbit polyclonal antibody to DYKDDDDK tag

Images & Validation

Tested ApplicationsWB
Dilution rangeWestern Blot 1:3000-1:5000 Immunofluorescence 1:500-1:1000

Key Properties

HostRabbit
ClonalityPolyclonal
IsotypeRabbit-IgG
ImmunogenSynthetic peptide (DYKDDDDK)
TargetDYKDDDDK Tag (same epitope as Sigma's Anti-FLAG M2)

Storage & Handling

StorageStore at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
DisclaimerFor research use only
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DYKDDDDK Tag (same epitope as Sigma's FLAG M2) Antibody

All lanes: Anti DYKDDDDK-tag antibody at 1/3000 dilution, Lysates/proteins at 35 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294275 overnight at 4°C.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

DYKDDDDK Tag (same epitope as Sigma's FLAG M2) Antibody (orb1294275)

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25 μl
$ 240.00
100 μl
$ 350.00
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