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Catalog Number | orb413729 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to DYKDDDDK Tag |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | All |
Immunogen | Synthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH). |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000) |
Conjugation | Unconjugated |
Target | DYKDDDDK Tag |
Storage | -20°C |
Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated |
Alternative names | DDDDK epitope tag antibody, DDDK antibody, ddk ant Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.
ICC, IF, IHC, WB | |
All | |
Rabbit | |
Polyclonal | |
RPE |
ICC, IF, IHC | |
All | |
Rabbit | |
Polyclonal | |
APC |
ELISA, ICC, IF, IHC, WB | |
All | |
Rabbit | |
Polyclonal | |
Biotin |
ICC, IF, IHC | |
All | |
Rabbit | |
Polyclonal | |
FITC |
ELISA, IHC, WB | |
All | |
Rabbit | |
Polyclonal | |
HRP |