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DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG M2 Antibody)

Catalog Number: orb413729

DispatchUsually dispatched within 2-3 weeks
$ 530.00
Catalog Numberorb413729
CategoryAntibodies
DescriptionRabbit polyclonal antibody to DYKDDDDK Tag
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsWB
ReactivityAll
ImmunogenSynthetic peptide corresponding to the DYKDDDDK Tag, conjugated to Keyhole Limpet Haemocyanin (KLH).
Concentration1 mg/ml
Dilution rangeWB (1:1000)
ConjugationUnconjugated
TargetDYKDDDDK Tag
Storage-20°C
Buffer/PreservativesPBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Alternative namesDDDDK epitope tag antibody, DDDK antibody, ddk ant
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NoteFor research use only
Application notesA 1:1000 dilution of SPC-720 was sufficient for detection of DYKDDDDK Tag in 15 µg of DYKDDDDK (FLAG) Positive Control Lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.
DYKDDDDK Tag Antibody (Equivalent to Sigma's Anti-FLAG M2 Antibody)

Dot Blot analysis using Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody. Tissue: DYKDDDDK (FLAG) Positive Control Lysate. Primary Antibody: Rabbit Anti-DYKDDDDK Tag Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. The quantities of protein spotted on each panel are as shown.