DYKDDDDK (FLAG) peptide

• Catalog Number: orb343849
• Category: Proteins
• Description: DYKDDDDK (FLAG) peptide
• Tested applications: IP, SDS-PAGE, WB
• Concentration: 1 mg/mL
• Dilution range: IP: User Optimized - see PROTOCOL, WB: User Optimized as blocking peptide in PCA
• Form/Appearance: Lyophilized
• Purity: Greater than 90% specific peptide by HPLC and MS.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: synthetic FLAG peptide, ddddk peptide, dykddddk pe
• Note: For research use only
• Application notes: The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Cell lysate before purification using DYKDDDDK (FLAG) peptide

Western blot analysis of DYKDDDDK tagged recombinant protein flow through wash using DYKDDDDK (FLAG) peptide

Western blot analysis of crude lysate containing over-expressed DYKDDDK-tagged recombinant protein using DYKDDDDK (FLAG) peptide