DYKDDDDK (FLAG) peptide

• Catalog Number: orb343849
• Category: Proteins
• Description: DYKDDDDK (FLAG) peptide
• Tested applications: IP, SDS-PAGE, WB
• Concentration: 1 mg/mL
• Dilution range: IP: User Optimized - see PROTOCOL, WB: User Optimized as blocking peptide in PCA
• Form/Appearance: Lyophilized
• Purity: Greater than 90% specific peptide by HPLC and MS.
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Buffer/Preservatives: None
• Alternative names: synthetic FLAG peptide, ddddk peptide, dykddddk pe
• Note: For research use only
• Application notes: The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAG® tag) recombinant protein (arrowhead). Load: (6 µl per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.

SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: crude lysate containing over-expressed DYKDDDK-tagged recombinant protein. Lane 2: unbound flow-through of consisting of endogenous E.coli proteins. Lane 3: enriched recombinant protein. Load: 5 µg protein, 15 µl flow through. Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.

SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction 1. Lane 5: eluted fraction 2. Lane 6: eluted fraction 3. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15 µl per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.