DYKDDDDK (FLAG) peptide

SKU: orb343849

Description

DYKDDDDK (FLAG) peptide

Images & Validation

• Tested Applications: IP, SDS-PAGE, WB
• Application Notes:
  The FLAG peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG peptide is typically used for competitive elution of FLAG containing proteins from anti-DYKDDDDK affinity gels. For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG affinity gel PROTOCOL for specific instructions for use. Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4. Reconstitution Volume: 1.0 mL

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Key Properties

• Conjugation: Unconjugated
• Purity: Greater than 90% specific peptide by HPLC and MS.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

synthetic FLAG peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG