DYKDDDDK (FLAG) peptide

SKU: orb343849

Description

DYKDDDDK (FLAG) peptide

Images & Validation

• Tested Applications: IP, SDS-PAGE, WB
• Application Notes:
  The FLAG® peptide was tested by SDS-PAGE. A working concentration of 500 μg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels. For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for specific instructions for use. Reconstitution Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4. Reconstitution Volume: 1.0 mL

Key Properties

• Purity: Greater than 90% specific peptide by HPLC and MS.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: None. Stabilizer: None. None
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

synthetic FLAG peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide, DDK, FLAG

SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: Cell lysate before purification. Lane 2: Flow through (used cell lysate). Lane 3: Purified DYKDDDDK (FLAG® tag) recombinant protein (arrowhead). Load: (6 µl per lane). Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.

SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel. Lane 1: crude lysate containing over-expressed DYKDDDK-tagged recombinant protein. Lane 2: unbound flow-through of consisting of endogenous E.coli proteins. Lane 3: enriched recombinant protein. Load: 5 µg protein, 15 µl flow through. Predicted/Observed size: 70kDa for DYKDDDDK tagged recombinant protein.

SDS-PAGE of Anti-DYKDDDDK affinity gel using DYKDDDDK control peptide. Lane 1: DYKDDDDK tagged recombinant protein. Lane 2: flow through. Lane 3: wash. Lane 4: eluted fraction 1. Lane 5: eluted fraction 2. Lane 6: eluted fraction 3. Lane 7: blank. Lane 8: molecular weight markers. Load: 5 µg protein lane 1, 15 µl per each other lane. Predicted/Observed size: 135kDa for DYKDDDDK tagged recombinant protein.