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Catalog Number | orb20489 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to DYDC1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Human, Mouse, Rat |
Dilution range | ELISA: 1:128000, WB: 0.1-1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 20.9 |
Target | DYDC1 (aa142-154) |
Entrez | 143241 |
Protein Sequence | TLAEISDRYGAPN |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti DYDC1 antibody, anti DPY30 domain containing Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 28kDa band in Rat Testis lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 20.9kDa according to Human NP_620167.1 and of 21.8kDa according to Rat XP_001066918.2. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HEK293 overexpressing Human DYDC1 lysate (Lane1), HEK293 mock-transfected lysate (Lane2), HEK293 overexpressing Human DYDC1 lysate (Lane3) using DYDC1 antibody
Immunohistochemical staining of Human Testis using DYDC1 antibody
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