DUOX1 Antibody

SKU: orb1248362

Description

DUOX1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 170kDa band observed in Human Testis lysates (calculated MW of 177kDa according to NP_059130.2). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CSRSDIETELTPQR
• Target: DUOX1
• Molecular Weight: Approx 170kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DUOX1, dual oxidase 1, LNOX1, MGC138840, MGC138841, NOXEF1, THOX1, NADPH thyroid oxidase 1, flavoprotein NADPH oxidase, large NOX 1, long NOX 1, nicotinamide adenine dinucleotide phosphate oxidase, thyroid oxidase 1

orb1248362 (0.1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

DUOX1

UniProt

Q9NRD9

RefSeq (Protein):NP_059130.2