DUOX1 Antibody

SKU: orb1247589

Description

DUOX1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human Placenta and Thyroid Gland lysates gave no specific signal but low background (at antibody concentration up to 0.5µg/mL). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Thyroid shows staining of membranes in epithelial cells of the gland. Recommended concentration, 4-6µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: EKFQRSCLHQTVQ
• Target: DUOX1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DUOX1 , dual oxidase 1 , LNOX1, MGC138840, MGC138841, NOXEF1, THOX1, NADPH thyroid oxidase 1, flavoprotein NADPH oxidase, nicotinamide adenine dinucleotide phosphate oxidase

orb1247589 (4 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Quick Database Links

Gene Symbol

DUOX1

UniProt

Q9NRD9

RefSeq (Protein):NP_059130.2, NP_787954.1