DTX3 Antibody

• Catalog Number: orb1249407
• Category: Antibodies
• Description: DTX3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-KNKVTVSKPVWD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DTX3
• UniProt ID: Q8N9I9
• NCBI: NP_848597.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DTX3, deltex 3 homolog (Drosophila), FLJ34766, MGC
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90kDa band observed in Mouse Fetal Brain lysates and in lysates of cell line NIH3T3 (calculated MW of 38.0kDa according to Human NP_848597.1 and 37.98kDa to Mouse NP_109639.1). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies (Takeyama et al, J Biol Chem. 2003 Jun13;278(24):21930-7.; PMID: 12670957). Recommended concentration: 0.2-0.5ug/ml.Immunohistochemistry:Paraffin embedded Human Brain (Cortex). Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249407 (0.3 ug/ml) staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249407 (10 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.