DRAP1 Antibody / NC2 alpha

• Catalog Number: orb1826054
• Category: Antibodies
• Description: NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2beta, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-DRAP1-1A8
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant human DRAP1 protein was used as the immunogen for the DRAP1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Conjugation: Unconjugated
• Formula: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Hazard Information: This DRAP1 antibody is available for research use only.
• UniProt ID: Q14919
• Storage: Aliquot the DRAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed human HeLa cells with DRAP1 antibody (clone PCRP-DRAP1-1A8) followed by goat anti-mouse IgG-CF488 (green); Red = CF640R phalloidin.

Flow cytometry testing of PFA-fixed human HeLa cells with DRAP1 antibody (clone PCRP-DRAP1-1A8) followed by goat anti-mouse IgG-CF488 (blue); Red = unstained cells.

IHC staining of FFPE human breast tissue with DRAP1 antibody (clone PCRP-DRAP1-1A8) HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free DRAP1 antibody (clone PCRP-DRAP1-1A8) as confirmation of integrity and purity.

Analysis of a HuProt (TM) microarray containing more than 19000 full-length human proteins using DRAP1 antibody (clone PCRP-DRAP1-1A8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.