DRAGON (RGMB) Antibody (C-term)

• Catalog Number: orb1936020
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB08951
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 47547 Da
• Target: This DRAGON (RGMB) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-406 amino acids from the C-terminal region of human DRAGON (RGMB).
• UniProt ID: Q6NW40
• NCBI: NP_001012779.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: RGM domain family member B, DRG11-responsive axona
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DRAGON (RGMB) Antibody (C-term) in K562 cell line lysates (35 ug/lane). DRAGON (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-DRAGON (RGMB) Antibody (C-term) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: mouse brain lysate. Lane 3: mouse cerebellum lysate. Lane 4: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.