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DRAGON (RGMB) Antibody (C-term)
Description
Images & Validation
−| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P - 1:100-500, WB - 1:1000 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 47547 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
| Disclaimer | For research use only |
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Western blot analysis of DRAGON (RGMB) Antibody (C-term) in K562 cell line lysates (35 ug/lane). DRAGON (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with DRAGON (RGMB) (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-DRAGON (RGMB) Antibody (C-term) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: mouse brain lysate. Lane 3: mouse cerebellum lysate. Lane 4: NCI-H460 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 48 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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DRAGON (RGMB) Antibody (C-term) (orb1936020)
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