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DPYSL5 Mouse Monoclonal Antibody
Catalog Number: orb783361
| Catalog Number | orb783361 |
|---|---|
| Category | Antibodies |
| Description | DPYSL5 Mouse Monoclonal Antibody |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | C3W1 |
| Tested applications | ICC, WB |
| Predicted Reactivity | Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG1,k |
| Immunogen | Recombinant human DPYL5. |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-4000, ICC/IF=1:25 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 65 kDa |
| Target | DPYSL5 |
| UniProt ID | Q9BPU6 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Collapsin response mediator protein 5; CRAM; CRMP Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Sample: Lane 1: SH-SY5Y cell lysates, Lane 2: Mouse brain tissue lysates, Lane 3: Rat brain tissue lysates, Primary: Anti-DPYSL5 (orb783361) at 1/4000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 65 kD.
SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (DPYSL5) monoclonal Antibody, Unconjugated (orb783361) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
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