Dopamine D5 receptor Rabbit Polyclonal Antibody

• Catalog Number: orb13365
• Category: Antibodies
• Description: Dopamine D5 receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Human
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat DRD5 (251-350/475aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: DRD5
• UniProt ID: P25115
• RRID: AB_10748703
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: D(1B) dopamine receptor; D(5) dopamine receptor; D
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: RSC96 (blue). Primary Antibody: Rabbit Anti-DRD5 antibody (orb13365), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Antibody (orb13365, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of orb13365 at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Brain (Mouse) Lysate at 40 ug, Testis (Mouse) Lysate at 40 ug, Primary: Anti-Dopamine D5 receptor (orb13365) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53kD, Observed band size: 53kD.

Sample: Skin (Mouse) Lysate at 40 ug, Skin (Rat) Lysate at 40 ug, Primary: Anti-Dopamine D5 receptor (orb13365) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 53 kD.