Donkey anti-Sheep IgG (H&L), ALP conjugated

• Catalog Number: orb699955
• Category: Antibodies
• Description: Donkey polyclonal antibody against Donkey anti-Sheep IgG (H&L) conjugated to AP
• Clonality: Polyclonal
• Species/Host: Donkey
• Conjugation: ALP
• Reactivity: Sheep
• Form/Appearance: Liquid
• Purity: Immunogen affinity purified donkey IgG.
• Purification: APL conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative
• Immunogen: Purified sheep IgG, whole molecule
• Tested applications: ELISA, ICC, IHC, WB
• Dilution range: 1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
• Application notes: No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
• Storage: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.