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Catalog Number | orb1289526 |
---|---|
Category | Proteins |
Description | Dog IgG protein |
Tested applications | IH, IP, WB |
Form/Appearance | Purified Dog IgG in PBS, pH 7.3, 0.01% sodium azide. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Source | Dog |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Purified Dog IgG in PBS, pH 7.3, 0.01% sodium azide. |
Note | For research use only |
Application notes | Please minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Dog IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Dog IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG and anti-Dog Serum. |
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Dog IgG whole molecule was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Dog IgG whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG and anti-Dog Serum. | |
Dog |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Pepsin. | |
Dog |
SDS-PAGE | |
Unconjugated | |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti- Papain. | |
Dog |