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Catalog Number | orb346963 |
---|---|
Category | Antibodies |
Description | Dog IgG F(ab')2 antibody (Biotin) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Canine |
Isotype | IgG |
Immunogen | Dog IgG F(ab')2 fragment |
Concentration | 2.0 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Lyophilized |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Dog IgG, Dog IgG F(ab’)2 and Dog Serum. No reaction was observed against Dog IgG F(c). |
Conjugation | Biotin |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Alternative names | rabbit anti Dog IgG F(ab')2 Antibody Biotin conjug Read more... |
Note | For research use only |
Application notes | Secondary antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry as well as other antibody detection methods. |
Expiration Date | 12 months from date of receipt. |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Dog IgG, anti-Dog IgG F(ab’)2 and anti-Dog Serum. No reaction was observed against anti-Dog IgG F(c) or anti-Pepsin. | |
Dog |
ELISA, IHC, WB | |
Canine | |
Rabbit | |
Polyclonal | |
Biotin |
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