Dnmt3b Rabbit Polyclonal Antibody

SKU: orb500943

Description

Dnmt3b Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Equine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Dnmt3b (251-350/853aa)
• Target: DNMT3B
• Molecular Weight: 94 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Cytosine 5methyltransferase 3B; DNA(cytosine 5) methyltransferase 3 beta; DNA; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; Dnmt3b; Dnmt3 beta; EC 2.1.1.37; ICF; M.HsaIIIB; MGC124407; RP23-89H14.3; DNM3B_HUMAN.

Blank control (black line): K562. Primary Antibody (green line): Rabbit Anti-Dnmt3b antibody (orb500943), dilution: 2 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control (blue line): HepG2 (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-Dnmt3b antibody (orb500943), dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-Dnmt3b (orb500943) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 94 kD, Observed band size: 80/94 kD.

Quick Database Links

Gene Symbol

DNMT3B

UniProt

Q9UBC3