Dnmt1 Rabbit Polyclonal Antibody

SKU: orb500550

Description

Dnmt1 Rabbit Polyclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Dnmt1 (1201-1300/1616aa)
• Target: DNMT1
• Molecular Weight: 178 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DNMT1_BOVIN; DNMT1; 2.1.1.37; DNMT1_HUMAN; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI (DNA MTase HsaI | M.HsaI); MCMT; AIM; CXXC9; DNMT; DNMT1_MOUSE; Met-1; DNA methyltransferase MmuI (DNA MTase MmuI | M.MmuI); Met1; Uim; DNMT1_RAT; DNA MTase RnoIP (M.RnoIP); DNA methyltransferase I;

Paraformaldehyde-fixed, paraffin embedded (Human stomach carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Dnmt1) Polyclonal Antibody, Unconjugated (orb500550) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

DNMT1

UniProt

P26358