DNASE2 Antibody

• Catalog Number: orb1239023
• Category: Antibodies
• Description: DNASE2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II.The immunogen is located within the last 50 amino acids of DNase II.
• Concentration: 1 mg/ml
• Dilution range: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Aprox: 40 kDa
• Target: DNASE2
• UniProt ID: O00115
• NCBI: AF047016
• Storage: DNase II antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DNase II Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonu
• Note: For research use only
• Application notes: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DNase II in human spleen tissue lysate in the absence (lane 1) or presence (lane 2) of blocking peptide with DNase antibody II at 1:500 dilution.

Immunohistochemistry of DNaseII in human spleen tissue with DNaseII antibody at 5 µg/mL.

Immunofluorescence of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml. Green: DNase II antibody (orb1239023) Red: Phylloidin staining Blue: DAPI staining.

Immunohistochemistry of DNase II in human spleen tissue with DNase II antibody at 5 µg/ml.