DNAH5 Rabbit Polyclonal Antibody

• Catalog Number: orb183142
• Category: Antibodies
• Description: DNAH5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Equine, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DNAH5 (4001-4200/4624aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 529 kDa
• Target: DNAH5
• UniProt ID: Q8TE73
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: axonemal; axonemal; Axonemal beta dynein heavy cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: A549. Primary Antibody (green line): Rabbit Anti-DNAH5/FITC Conjugated antibody, dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.