DNA-PKcs Antibody

• Catalog Number: orb1523843
• Category: Antibodies
• Description: DNA-PKcs Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 4078 and 4128
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DNA-PKcs
• UniProt ID: P78527
• NCBI: NP_008835.5
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: hyper-radiosensitivity of murine scid mutation, co
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DNA-PKcs by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified goat anti-DNA-PKcs antibody orb1523843 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human DNA-PKcs by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-DNA-PKcs antibody orb1523843 used for IP at 6 µg/mg lysate.

Detection of human DNA-PKcs by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-DNA-PKcs (Cat. No. orb1523843) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB