λDNA/ HindIII Digest

• Catalog Number: orb1734268
• Category: Tools
• Description: The HindIII digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125. The cohesive ends (12b cos site of bacteriophage lambda) of fragments 23130 bp and 4361 bp may be separated by heating to 65 °C for 5 min.
• Form/Appearance: liquid, DNA fragment mix in 10 mM Tris-HCl pH 7.5, 10 mM EDTA, 8% (w/v) Glycerol, Tartrazine and Xylene Cyanol FF; Color: green
• Concentration: 100 ng/µl
• Application notes: < b>Preparation: Lambda DNA (48.502 bp) was completely digested by HindIII, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. < br>< b>Recommended Load: 5 µl per lane.
• Storage: store at -20 °C. Short term storage (up to 1 month) at 4 °C possible
• Note: For research use only