DMPO antibody

• Catalog Number: orb147945
• Category: Antibodies
• Description: Mouse monoclonal to DMPO (RPE). The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems (2-6). Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N1664A
• Tested applications: AM, ELISA, ICC, IHC, IP, WB
• Reactivity: All
• Isotype: IgG1
• Immunogen: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100), ELISA (1:100), IP (25µg)
• Conjugation: RPE
• Target: DMPO
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: 5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxi
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-189 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.