DMBT1 Rabbit Polyclonal Antibody

SKU: orb578441

Description

Rabbit polyclonal antibody to DMBT1

Research Area

Disease Biomarkers, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Predicted Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DMBT1
• Target: DMBT1
• Molecular Weight: 258kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

SAG, GP340, SALSA, muclin

Sample Tissue: Human HepG2 Whole Cell, Antibody Dilution: 3 ug/ml.

Sample Tissue: Human Human Stomach, Antibody Dilution: 1 ug/ml.

Positive control (+): Human Lung (LU), Negative control (-): HeLa Cell Lysate (HL), Antibody concentration: 1 ug/ml.

Lanes: Lane 1: 2 ug MCF7-DMBT1+Dox, Lane 2: 2 ug MCF7-DMBT1 -Dox, Lane 3: 2 ug MCF7-Ctrl+Dox, Lane 4: 2 ug MCF7-DMBT1-Dox, Primary Antibody Dilution: 1:5000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:10000, Gene Name: DMBT1.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-DMBT1 Antibody Titration: 0.2-1 ug/ml, Positive Control: Human Stomach.

Quick Database Links

Gene Symbol

DMBT1

UniProt

Q9UGM3

RefSeq (Protein):NP_015568