DiMethyl-Histone H3-K9 pAb

• Catalog Number: orb1258189
• Category: Antibodies
• Description: DiMethyl-Histone H3-K9 pAb
• Target: H3K9me2
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Other, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: A synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
• UniProt ID: Q16695
• MW: Observed: 18kDa
• Tested applications: ChIP, IF, IHC, IP, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200ChIP: 1:50 - 1:200CHIP seq: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H3F3A, H3t, H3.4, H3/g, H3FT
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K9 antibody (orb1258189). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K9 antibody (orb1258189).

Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K9 antibody (orb1258189) at dilution of 1:200 (40x lens).

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K9 antibody (orb1258189). Blue: DAPI for nuclear staining.

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K9 antibody (orb1258189) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.