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Catalog Number | orb215929 |
---|---|
Category | Antibodies |
Description | Dihydrofolate reductase (DHFR) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, RatImmunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 21453 MW |
UniProt ID | P00374 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Dihydrofolate reductase;1.5.1.3;DHFR; Read more... |
Note | For research use only |
Application notes | WB: The detection limit for DHFR is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-DHFR antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of DHFR using anti-DHFR antibody.Lane 1:human HeLa cell; 2:human Jurkat cell; 3:rat liver tissue; 4:rat kidney tissue; 5:mouse liver tissue; 6:mouse kidney tissue.
IF analysis of DHFR using anti- DHFR antibody. DHFR was detected in immunocytochemical section of U20S cells.
IHC analysis of DHFR using anti-DHFR antibody. DHFR was detected in a paraffin-embedded section of mouse brain tissue.
IHC analysis of DHFR using anti-DHFR antibody. DHFR was detected in a paraffin-embedded section of rat brain tissue.
IHC analysis of DHFR using anti-DHFR antibody. DHFR was detected in a paraffin-embedded section of human lung cancer tissue.
ELISA, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC, WB | |
Human, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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