DHFR Antibody (monoclonal, 3C8)

• Catalog Number: orb527060
• Category: Antibodies
• Description: DHFR Antibody (monoclonal, 3C8)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3C8
• Tested applications: IHC, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFRshares90%aminoacid (aa)sequenceidentitywith both mouseandrat DHFR.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22 kDa
• UniProt ID: P00374
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Dihydrofolate reductase; DHFR
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of DHFR using anti-DHFR antibody.Lane 1:human K562 cell;2:human SGC-7901 cell;3:human HeLa cell;4:human SW620 cell;5:rat kidney tissue.

IHC analysis of DHFR using anti-DHFR antibody. DHFR was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of DHFR using anti-DHFR antibody. DHFR was detected in paraffin-embedded section of rat gaster tissue.