DIAPH3 Rabbit Polyclonal Antibody

SKU: orb183107

Description

DIAPH3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DIAPH3 (801-900/1193aa)
• Target: DIAPH3
• Molecular Weight: 137 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AN; AUNA1; Dia2; diap3; DIAP3_HUMAN; DIAPH3; Diaphanous homolog 3(Drosophila); Diaphanous homolog 3; Diaphanous related formin 3; Diaphanous-related formin-3; DKFZp434C0931; DKFZp686A13178; DRF3; FLJ34705; mDia2; NSDAN; OTTHUMP00000018480; Protein diaphanous homolog 3; RP11-26P21.1.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DIAPH3) Polyclonal Antibody, Unconjugated (orb183107) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DIAPH3) Polyclonal Antibody, Unconjugated (orb183107) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

DIAPH3

UniProt

Q9NSV4