DIA-2 antibody

• Catalog Number: orb345489
• Category: Antibodies
• Description: DIA-2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.
• Concentration: 1.4 mg/mL
• Dilution range: ELISA: 1:12,000 - 1:60,000, IP: 1:100, WB: 1:1,000 - 1:6,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human DIA-2. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human based on 100% homology for the immunogen sequence. However, cross reactivity is expected with mouse DIA-2 based on a 93% homology to the immunogen sequence. Cross reactivity with DIA-2 homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: O60879
• NCBI: 5803003
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-DIA-2 Antibody, DIA 2, DIA drome, DIA2
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 125 kDa in size corresponding to DIA-2 by western blotting in the appropriate cell lysate or extract. Alternate splice variants have been described for this protein. This antibody will only react with DIA-156 (isoform 1 of DIA-2).
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HEK293 cells using DIA-2 antibody