DFNA5 Rabbit Polyclonal Antibody

• Catalog Number: orb183072
• Category: Antibodies
• Description: DFNA5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Human, Sheep
• Reactivity: Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DFNA5 (21-120/496aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: GSDME
• UniProt ID: O60443
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 2310037D07Rik; 4932441K13Rik; Deafness, autosomal
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Protein: intestinal (mouse) lysate at 40 ug, Primary: rabbit Anti-DFNA5 (orb183072) at 1:300, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Small intestine (Mouse) Lysate at 40 ug, Primary: Anti-DFNA5 (orb183072) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Paraformaldehyde-fixed, paraffin embedded (rat spleen tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DFNA5) Polyclonal Antibody, Unconjugated (orb183072) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.