Dffb Antibody

• Catalog Number: orb1239262
• Category: Antibodies
• Description: Dffb Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Human, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: CAD antibody was raised against a 16 amino acid peptide near the carboxy terminus of murine CAD.The immunogen is located within the last 50 amino acids of CAD.
• Concentration: 1 mg/ml
• Dilution range: CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 40 kDa
• Target: Dffb
• UniProt ID: O54788
• NCBI: O54788
• Storage: CAD antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CAD Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: CAD Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 5730
• Note: For research use only
• Application notes: CAD antibody antibody can be used for detection of CAD by Western blot at 0.5 μg/mL. A 40 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CAD in mouse lung (L) and kidney (K) tissue lysates with CAD antibody at 1:500 dilution.

Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 1 µg/mL.

Immunofluorescence of CAD in Mouse Kidney cells with CAD antibody at 10 µg/mL.

Immunohistochemistry of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml.

Immunofluorescence of CAD in mouse kidney tissue with CAD antibody at 5 µg/ml. Green: CAD antibody (orb1239262) Red: Phylloidin staining Blue: DAPI staining.