Dffb Antibody

• Catalog Number: orb1239028
• Category: Antibodies
• Description: Dffb Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: DFF40 antibody was raised against a peptide corresponding to 18 amino acids near the center of murine CAD.The immunogen is located within amino acids 130 - 180 of DFF40.
• Concentration: 1 mg/mL
• Dilution range: DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 38 kDa Observed: 40 kDa
• Target: Dffb
• UniProt ID: O54788
• NCBI: NP_031885
• Storage: DFF40 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: DFF40 Antibody: CAD, CPAN, 40kDa, DFF40, Didff, 57
• Note: For research use only
• Application notes: DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 μg/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.

Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 µg/mL.

Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/ml.

Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 µg/mL. Green: DFF40 Antibody (orb1239028) Blue: DAPI staining.