Dextranase Antibody

SKU: orb344578

Description

Dextranase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Algae
• Application Notes:
  DEXTRANASE Antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Dextranase [Penicillium species] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Dextranase [Penicillium species]
• Purity: Anti-DEXTRANASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species]. Cross reactivity against Dextranase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Disclaimer: For research use only

Alternative Names

rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX

Both the antiserum and IgG fractions of anti-Dextranase (Penicillium) are shown to detect under reducing conditions of SDS-PAGE the 41000 dalton enzyme in cellular extracts. Approximately 10 ug of total protein is loaded per lane. A 1:2000 dilution of the primary antibody is used followed by detection using HRP Goat-a-Rabbit IgG [H&L] (orb347673) diluted 1:4000 and color development using 4-CN substrate until sufficient color develops. Other detection systems will yield similar results.

Quick Database Links

UniProt

P48845

RefSeq:AAB47720.1