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Detection of FLAG proteins antibody

Catalog Number: orb345396

DispatchUsually dispatched within 5-10 working days
$ 453.00
Catalog Numberorb345396
CategoryAntibodies
DescriptionDetection of FLAG proteins antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
IsotypeIgG
ImmunogenThis antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. This antibody reacts with FLAG conjugated proteins.
Concentration1.13mg/mL
Dilution rangeELISA: 1:20,000 - 1:40,000, IHC: User Optimized, WB: 1:2,000 - 1:10,000
Form/AppearanceLiquid (sterile filtered)
PurityThis affinity purified antibody is directed against the FLAG motif and is useful in determining its presence in various assays. This polyclonal anti-FLAG tag antibody detects over-expressed proteins containing the FLAG epitope tag. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
ConjugationUnconjugated
StorageStore vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesrabbit antibody for the detection of FLAG conjugat
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NoteFor research use only
Application notesThis antibody is optimally suited for monitoring the expression of FLAG tagged fusion proteins. As such, this antibody can be used to identify fusion proteins containing the FLAG epitope. The antibody recognizes the epitope tag fused to either the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and FLAGä containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation, immunocytochemistry, and other immunodetection techniques. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK. Rockland Immunochemical's polyclonal antibody to detect FLAG conjugated proteins binds FLAG containing fusion proteins with greater affinity than the widely used monoclonal M1, M2 and M5 clones, and shows greater sensitivity in most assays. Affinity purification of the polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
Expiration Date12 months from date of receipt.

Western blot analysis of shown to detect as little as 3 ng of amino-terminal FLAG tagged recombinant protein using Detection of FLAG proteins antibody

Western blot analysis of detect FLAG conjugated proteins detects both C terminal linked and N terminal linked FLAG tagged recombinant proteins using Detection of FLAG proteins antibody

Western Blot analysis of Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: CHO/K1 Lysate. Lane 5: A431 Lysate Lane 6: Jurkat Lysate. Lane 7: NIH/3T3 Lysate. Lane 8: E-coli HCP Control. Lane 9: FLAG Positive Control Lysate. Lane 10: Red Fluorescent Prote

Western Blot analysis of of Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: FLAG Positive Control Lysate(10ug) [+]. Lane 3: 12 Epitope GST Tagged Lysate (10ug) [+]. Lane 4: rGST protein (0.1µg) [-]. Lane 5: MBP protein (0.1ug) using Detection of

  • Flag Tag antibody [orb129610]

    ICC,  IF,  IHC-P,  IP,  WB

    Other

    Mouse

    Monoclonal

    Unconjugated

    100 μl, 50 μl, 200 μl
  • Detection of FLAG proteins antibody [orb345397]

    ELISA,  IHC,  WB

    Rabbit

    Polyclonal

    Unconjugated

    25 μl
  • DYKDDDDK antibody (Biotin) [orb345881]

    ELISA,  IHC,  WB

    Rabbit

    Polyclonal

    Biotin

    100 μg
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