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Product Overview
Product Name Flag Tag antibody
Catalog Number orb129610
Species/HostMouse
ReactivityOther
ConjugationUnconjugated
Tested applicationsICC, IF, IHC-P, IP, WB
Immunogen A synthetic peptide DYKDDDDK coupled to KLH.
Target FLAG Tag
Alternative Names
Product Properties
Form/Appearance Mouse IgG1 in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration 20 μg: 0.2 mg/ml
50 μg: 0.5 mg/ml
100 μg: 1mg/ml
200 μg: 1mg/ml
Storage At -20°C for one year. After reconstitution at 4°C for two weeks. It can also be aliquotted and stored frozen at -20°C for a longer time.
Note For research use only.
Isotype Mouse IgG1
ClonalityMonoclonal
Purity Affinity chromatography
Product Description

Mouse monoclonal to Flag Tag.FLAG tag Mouse mAb is part of the series of Tag antibodies, the excellent quality in the research. FLAG tag antibody is a highly sensitive and affinity PAB applicable to FLAG tagged fusion protein detection. FLAG tag antibody can detect FLAG tags in internal, C terminal, or N terminal recombinant proteins.

Application Notes
Dilution Range WB: 1:3000-1:10000, IF:1:200
Validation Images
Western blot analysis of HEK 293 cell lysate using FLAG Tag antibody. Flag-Tag Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with FLAG-tag protein C: IP (PBS + anti- FLAG mAb 4℃ overnight) D: IP (H
Western blot analysis of HEK 293 cell lysate using FLAG Tag antibody. Flag-Tag Mouse Monoclonal antibody A: HEK 293 cell lysate without transfected B: HEK 293 cell lysate transfected with FLAG-tag protein C: IP (PBS + anti- FLAG mAb 4℃ overnight) D: IP (H
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