Desmoplakin I+II Rabbit Polyclonal Antibody

SKU: orb13377

Description

Desmoplakin I+II Rabbit Polyclonal Antibody

Research Area

Signal Transduction, Transcription Regulators, desmosomes

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Gallus, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DP-I (261-360/2871aa)
• Target: DSP
• Molecular Weight: 333 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Desmoplakin I; 250/210 kDa paraneoplastic pemphigus antigen; Desmoplakin(DPI DPII); Desmoplakin; Desmoplakin II; DP; DP I; DP II; DPI; DP-I; DPII; DSP; PPKS 2; PPKS2; DESP_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Desmoplakin I+II) Polyclonal Antibody, Unconjugated (orb13377) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

DSP

UniProt

P15924