Desmin Rabbit Polyclonal Antibody (FITC)

SKU: orb15501

Description

Desmin Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: ICC, IF
• Dilution range: ICC/IF=1:50-200, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Desmin (261-360/470aa)
• Target: DES
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

DESM_HUMAN; DES;

Blank control: H9C2. Primary Antibody (green line): Rabbit Anti-Cardiac Troponin T antibody (orb15501), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Tissue/Cell: H9C2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal rabbit serum) at 37°C for 20 min, Incubation: Anti-Desmin Polyclonal Antibody, conjugated (orb15501) 1:50, 2 hours at 37°C, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

DES