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Catalog Number | orb1525885 |
---|---|
Category | Antibodies |
Description | Derlin-1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 201 and 251 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Derlin-1 |
UniProt ID | Q9BUN8 |
NCBI | NP_077271.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Der1-like protein 1; derlin-1; Der1-like domain fa Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse Derlin-1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Derlin-1 antibody orb1525885 used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 3 µg/mg lysate. Derlin-1 was also immunoprecipitated by rabbit anti-Derlin-1 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Detection of human Derlin-1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Derlin-1 (Cat. No. orb1525885) used at a dilution of 1: 200 (1µg/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
IHC-P | |
Bovine, Canine, Equine, Mouse, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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