DERL1 Antibody

• Catalog Number: orb1262529
• Category: Antibodies
• Description: DERL1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This DERL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the C-terminal region of human DERL1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 29 kDa
• Target: DERL1
• UniProt ID: Q9BUN8
• NCBI: Q9BUN8
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Derlin-1, Degradation in endoplasmic reticulum pro
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:16000 dilution + SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of DERL1 Antibody in mouse NIH-3T3 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with DERL1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.