Defensin beta 2 Rabbit Polyclonal Antibody

SKU: orb10531

Description

Defensin beta 2 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from rat beta 2 Defensin (21-71/71aa)
• Target: Defb2
• Molecular Weight: 8 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

beta-defensin 2 precursor; beta Defensin 2; beta-defensin 2; Defb2; Q32ZI5_RAT; DFB4A_HUMAN.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-beta 2 Defensin Polyclonal Antibody, Unconjugated (orb10531) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Defb2

References

Leopoldo Cosme-Silva a 1 , Renan Dal-Fabbro b 1 , Fernanda de Lima Pontes a , Leticia Cabrera Capalbo c , Edilson Ervolino d , Luciano Tavares Angelo Cintra d , Juan José Segura-Egea e , João Eduardo Gomes Filho Multi-Strain Probiotic Formula Modulates Expression of β-Defensin-2, β-Defensin-3, and TLR-4 in Male Rats with Apical Periodontitis Archives of Oral Biology, (2024)

PubMed:

Applications

IHC

Reactivity

Rat

Effects of Bdellovibrio bacteriovorus HD100 on experimental periodontitis in rats Mol Oral Microbiol, 38, 158-170 (2023)

PubMed: 36495122