DDX6 monoclonal antibody (M01), clone 3D2

• Catalog Number: orb2293681
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant DDX6.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D2
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: DDX6 (AAH65007, 1 a.a. ~ 483 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MSTARTENPVIMGLSSQNGQLRGPVKPTGGPGGGGTQTQQQMNQLKNTNTINNGTQQQAQSMTTTIKPGDDWKKTLKLPPKDLRIKTSDVTSTKGNEFEDYCLKRELLMGIFEMGWEKPSPIQEESIPIALSGRDILARAKNGTGKSGAYLIPLLERLDLKKDNIQAMVIVPTRELALQVSQICIQVSKHMGGAKVMATTGGTNLRDDIMRLDDTVHVVIATPGRILDLIKKGVAKVDHVQMIVLDEADKLLSQDFVQIMEDIILTLPKNRQILLYSATFPLSVQKFMNSHLQKPYEINLMEELTLKGVTQYYAYVTERQKVHCLNTLFSRLQINQSIIFCNSSQRVELLAKKISQLGYSCFYIHAKMRQEHRNRVFHDFRNGLCRNLVCTDLFTRGIDIQAVNVVINFDFPKLAETYLHRIGRSGRFGHLGLAINLITYDDRFNLKSIEEQLGTEIKPIPSNIDKSLYVAEYHSEPVEDEKP
• NCBI: AAH65007
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

DDX6 monoclonal antibody (M01), clone 3D2 Western Blot analysis of DDX6 expression in Hela.

Immunoperoxidase of monoclonal antibody to DDX6 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]

Western Blot analysis of DDX6 expression in transfected 293T cell line by DDX6 monoclonal antibody (M01), clone 3D2. Lane 1: DDX6 transfected lysate (53.2 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of DDX6 over-expressed 293 cell line, cotransfected with DDX6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DDX6 monoclonal antibody (M01), clone 3D2 (Cat # orb2293681). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (78.87 KDa).