DDX6 Antibody

• Catalog Number: orb1530165
• Category: Antibodies
• Description: DDX6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 425 and 483
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:25,000 - 1:50,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: DDX6
• UniProt ID: P26196
• NCBI: NP_004388.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RN
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human DDX6 by western blot.Samples: Whole cell lysate from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells. Antibody: Affinity purified rabbit anti-DDX6 antibody orb1530165 at 0.04 µg/ml.

Detection of mouse DDX6 by western blot.Samples: Whole cell lysate from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells. Antibody: Affinity purified rabbit anti-DDX6 antibody orb1530165 at 0.04 µg/ml.

Detection of human DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human DDX6 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody.