DDX6 Antibody

• Catalog Number: orb1247612
• Category: Antibodies
• Description: DDX6 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-AEYHSEPVEDEKP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: DDX6
• UniProt ID: P26196
• NCBI: NP_004388.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: DEAD-box protien 6, DEAD (Asp-Glu-Ala-Asp) box pol
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Thymus. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247612 (0.3 ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247612 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247612 (5 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.