DDX3 Rabbit Polyclonal Antibody

SKU: orb1152009

Description

DDX3 Rabbit Polyclonal Antibody

Research Area

Cell Signaling, Intercellular Signaling, Molecular Biology, Surface Molecules

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DDX3 (1-100/662aa)
• Target: DDX3X
• Molecular Weight: 73 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAP-Rf; DBX; DDX14; DDX3; HLP2; MRX102; MRXSSB; D1Pas1-rs2; Fin14; DDX3X_HUMAN; DDX3X; DEAD box protein 3, X-chromosomal; DEAD box, X isoform (DBX); Helicase-like protein 2 (HLP2); 3.6.4.13; DDX3X_MOUSE; D1Pas1-related sequence 2; DEAD box RNA helicase DEAD3 (mDEAD3); Embryonic RNA helicase; Dead3; Erh;

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DDX3) Polyclonal Antibody, Unconjugated (orb1152009) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DDX3) Polyclonal Antibody, Unconjugated (orb1152009) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (DDX3) Polyclonal Antibody, Unconjugated (orb1152009) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

DDX3X

UniProt

O00571