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Catalog Number | orb1527012 |
---|---|
Category | Antibodies |
Description | DDX27 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human, Mouse |
Immunogen | between 700 and 750 |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended.; IP - 5 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DDX27 |
UniProt ID | Q96GQ7 |
NCBI | NP_060365.6 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | HSPC259; RNA helicase-like protein; RHLP; probable Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human DDX27 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX27 antibody orb1527012 used for IP at 10 µg/mg lysate.
Detection of human and mouse DDX27 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-DDX27 (Cat. No. orb1527012) used at a dilution of 1: 5, 000 (0.2µg/ml) and 1: 1, 000 (1µg/ml). Detection: DAB
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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