DCX/Doublecortin Rabbit Polyclonal Antibody

• Catalog Number: orb500968
• Category: Antibodies
• Description: DCX/Doublecortin Rabbit Polyclonal Antibody
• Target: DCX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human DCX (311-400/441aa)
• UniProt ID: O43602
• MW: 49 kDa
• Tested applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Doublecortex; DBCN; Dbct; DC; Doublin; Lis X; Liss
• Note: For research use only

Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 ug, Lane 2: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-DCX/Doublecortin (orb500968) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

SHSY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (DCX/Doublecortin) polyclonal Antibody, Unconjugated (orb500968) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.